Fabrication and use of the dual-flow-RootChip for the imaging of Arabidopsis roots in asymmetric microenvironments.

Stanley C., Shrivastava J., Brugman R. , Heinzelmann E. , Frajs V. , Bühler A. , van Swaay D., Grossmann G.

Fabrication and use of the dual-flow-RootChip for the imaging of Arabidopsis roots in asymmetric microenvironments.

Bio-Protocol, 8, (18), 2018, 1-19.

This protocol provides a detailed description of how to fabricate and use the dual-flowRootChip (dfRootChip), a novel microfluidic platform for investigating root nutrition, root-microbe interactions and signaling and development in controlled asymmetric conditions. The dfRootChip was developed primarily to investigate how plants roots interact with their environment by simulating environmental heterogeneity. The goal of this protocol is to provide a detailed resource for researchers in the biological sciences wishing to employ the dfRootChip in particular, or microfluidic devices in general, in their laboratory.

ISSN Print: 2331-8325
Digital Object Identifier (DOI): https://doi.org/10.21769/BioProtoc.3010
Publication-ID (Web Code): 40319 Sending by e-mail

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