Earthworms play a key role in regulating soil ecosystem services by burrowing through the soil, altering its structure, and making nutrients more available to plants. Conversely, earthworms are affected by soil properties often causing them to be absent in soils where they could have the greatest beneficial effect on soil structure and nutrient availability. The amendment of arable soils with organic matter (OM) is widely used as a tool to improve soil properties. However, it remains unclear how different types of OM affect earthworm burrowing activities and spatial burrowing patterns. We will present an experimental set-up for studying how different types of organic matter alter bioturbation behaviours of earthworms and its implications for soil property changes. Endogeic (burrower) earthworms will be introduced into soil columns amended with different types of OM commonly applied to arable soils (straw, manure, compost, cover crops). The combined effect of earthworms and OM additions on soil structure will then monitored over time by using non-destructive imaging techniques. Effects on nutrient availability will be monitored by destructive sampling at each timepoint. We expect that earthworm burrowing activity and its impact on soil pore structure establishment will vary with OM type due to direct effects on earthworms and indirect effects on soil properties. The results will support identifying targeted measures to accelerate earthworm burrowing and its beneficial effects on soil restoration.