Agroforestry for forage production is a promising solution to provide additional tree-based forage during drought periods in grasslands. However, information is lacking on the ecophysiological strategies of different tree species to acclimate to drier and warmer conditions and their impacts on fodder yield and quality. To address this, we planted hedgerows with six fodder tree species representing a broad range of isohydricity at six sites with different pedo-climatic conditions. Three years after the plantation, we measured ecophysiological responses (e.g., stomatal conductance, leaf water potential) and leaf biomass for each species. We also assessed the leaf chemical composition, which relates directly to palatability in livestock. We expect tree species that employ an anisohydric strategy (i.e., smoother stomatal control) to maintain leaf production with higher nutritive value and palatability for livestock. Altogether, our findings will shed light on the linkages between ecophysiological responses and fodder yield and quality in summer.