Thirty-one isolates of Lactobacillus parabuchneri were obtained from cheese containing histamine; of
these, 26 were found to possess the hdcA gene encoding histidine decarboxylase. By analysing the
genome data of 13 isolates, specific targets for the development of PCR-based detection and typing
systems for L. parabuchneri were identified. The real-time PCR for detection showed a linear quantification
over a range of 7 logs and a detection limit of 10 gene equivalents per reaction. The strain typing
method utilised the amplification of repeat sequences and showed discrimination comparable with a
phylogenetic tree, based on genome comparisons. The method was suitable for detecting and monitoring
the development of L. parabuchneri in raw milk and cheese.