Louis L., Angeli N., Pires J., Zahariev A., Chery I., Hossann C., Delavaud A., De La Torre A., Lerch S.
Deuterium oxide elimination kinetics for estimation of body water mass and flux: Developments for a thrifty, rapid and non-invasive method in dairy goats.
Dans: Animal - science proceedings. 12 - 15 September, Ed. 7th EAAP International Symposium on Energy and Protein Metabolism and Nutrition (ISEP 2022), Grenada, Spain. 2022, 473-474.
The in vivo deuterated water (D2O) elimination kinetics is a precise method to estimate body water mass, turnover and intake, and body chemical composition (Al-Ramamneh et al., 2010). Nonetheless, the reference methodology in ruminants requires intravenous (iv) injection of D2O followed by serial blood sampling, water cryoextraction and deuterium enrichment analysis by isotope-ratio mass spectrometry (IRMS). The aim was to investigate non-invasive sampling (milk vs. blood), fast water extraction (centrifugation vs. cryoextraction) and cheaper analysis method [isotope-ratio infrared spectroscopy (IRIS) vs. IRMS] to determine D2O elimination kinetics in dairy goats.
