Entomopathogenic fungi of the genera Metarhizium and Beauveria are important natural regulators of arthropod populations. To investigate their distribution and ecological drivers in real-world permanent grasslands, we did a survey analyzing fungal communities across 72 sites in Switzerland using ITS2 amplicon sequencing. We identified 10 Metarhizium and six Beauveria amplicon sequence variants (ASVs), with at least one ASV present at every site. Frequent ASVs included M. robertsii/M. pingshaense, M. brunneum, B. bassiana, and B. brongniartii. These ASVs showed distinct presence/absence and abundance patterns, indicating adaptation to climatic, soil physicochemical, and management-related factors. Factors such as soil pH, available phosphorus, temperature, bulk soil density, and management intensity significantlysignificantlysignificantlycorrelated with ASV distributions. For example, M. robertsii/M. pingshaense was widely distributed but strongly associated with soil pH, while B. brongniartii was restricted to subalpine regions, correlating with lower temperatures and nutrient levels. Plant communities, plant community-derived ecological indicator values (EIVs) reflectingreflectingreflectingenvironmental conditions, and indicator plant species analysis showed correlations with specific ASVs. On one hand, this suggests shared ecological niches and/or indicates possible direct plant–fungal interactions, consistent with the known endophytic or root-associated lifestyles of some entomopathogenic fungi. On the other hand, it suggests that plant-derived EIVs and individual indicator plant species may serve as effective indices for identifying habitats suitable for the presence and establishment of these entomopathogenic fungi. This approach may facilitate the evaluation of the suitability of particular environments for the application of Metarhizium and Beauveria strains as biological control agents.