The objective was to analyze the phylogeny and morphology of the Glomeromycetes on the order level hitherto consisting of Glomerales, Entrophosporales, Diversisporales and Gigasporales. The updated data sets show that Pacisporaceae is near to Gigasporales, but without support to place this family in Gigasporales or Diversisporales, while the Acaulosporaceae and Sacculosporaceae clades clearly separate from Diversisporaceae. The phylogeny indicates the transfer of Pacisporaceae from the Diversisporales to the new order Pacisporales. Pacisporales differ from all families in Gigasporales by type of spore formation, as forming spores on subtending hyphae instead on suspensors, by formation of vesicular-arbuscular mycorrhiza, and by germination characteristics. The phylogenetic analysis supports the separation of Acaulosporaceae and Sacculosporaceae from Diversisporales, from each other, and from all other Glomeromycetes orders. These two families are transferred to Acaulosporales and Sacculosporales. Acaulosporales are characterized by spore formation in a clear distance from globose termini of sporiferous saccules, three spore walls and generally by the diagnostic, ‘beaded’ inner wall. In Sacculosporales, there is no distance between the bi-walled spores and saccule termini, and the inner wall is never ‘beaded’. Additionally, four new families are separated within Glomerales, and one new family within Diversisporales.